Novel Chiral Stationary Phases for HPLC

Maher, Kevin (1994) Novel Chiral Stationary Phases for HPLC. Doctoral thesis, University of Manchester Institute of Science and Technology.

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Several silica-based chiral stationary phases (CSP) for HPLC were synthesised from an optically active carbohydrate, δ-gluconolactone. The separating abilities of these hydroxylated, acetylated and carbamated CSP were evaluated using test racemates that generally contained the electron-deficient dinitrobenzoyl group. Some insight into the chiral recognition process operating on the CSP was obtained by chromatographing fifty structurally related racemates. The effect on separating ability of "end-capping" the residual silanols on the silica support and of changing the way in which the organic strands were bonded within the CSP was also examined. The test racemates were resolved at different temperatures. Thermodynamic parameters that govern the complexation of the sample enantiomers with the CSP were derived from the resulting Van't Hoff plots. The CSP were analysed using solid-state NMR spectroscopy and secondary ion mass spectrometry. These two techniques provided evidence for the organic structures bonded to silica.

Item Type: Thesis (Doctoral)
Keywords: Chemistry
Depositing User: RED Unit Admin
Date Deposited: 08 Jul 2021 13:39
Last Modified: 09 Jul 2021 07:22

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